大乐透复式计算器

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0510-81608116

甲基化检测MSP

1)技术简介:DNA甲基化是哺乳类动物一个重要的基因外遗传信号。有研究表明,DNA甲基化与大多数肿瘤的发生相关,抑癌基因启动子CpG岛的高度甲基化可引起抑癌基因表达沉默,细胞出现无节制生长,导致肿瘤的发生。目前,基因的甲基化研究主要结合亚硫酸氢钠处理和PCR技术,分为甲基化特异性PCR(Methylation specific PCR,MSP)和硫化测序PCR(Bisulfite sequencing PCR, BSP)。

2)实验步骤/流程图:

甲基化特异性的PCRMSP原理:

    SHUANGLIANDNABIANXINGJIELIANHOU,ZAIHSO3-ZUOYONGXIAFASHENGC→UZHUANHUA,CRUOYIYOUJIAJIHUAZEWUCIGAIBIAN;JIAJIHUAXIUSHIZHIFASHENGYU5′→3′FANGXIANGC-GXIANGLIANJIEGOUDECSHANG,YINCIZAIHSO3-ZUOYONGHOU,DNA CpGDAORUOWUJIAJIHUA,ZEXULIEZHONGDEGAIBIANWEIC→U,CG→UG,RUOYOUJIAJIHUAZEWEIC→U,CG→CG,YONGBUTONGDEYINWUZUOPCR,JIKEJIANCECHUZHEIZHONGCHAYI,CONGERQUEDINGJIYINYOUWUCpGDAOJIAJIHUA。CONGLILUNSHANGSHUO,DNAFASHENGJIAJIHUADEMSPCHANWUDEXULIEYUYUANXULIEBIJIAO,BIANHUAWEIC→T,CG→CG,XIANGYINGQIHUBULIANDEGAIBIANWEIG→A,CG→CG。RANHOUSHEJIZHENDUIJIAJIHUAHEFEIJIAJIHUAXULIEDEYINWUBINGJINXINGJUHEMEILIANFANYING(PCR)KUOZENG,ZUIHOUTONGGUOQIONGZHITANGNINGJIAODIANYONGFENXI,QUEDINGYUYINWUHUBUDEDNAXULIEDEJIAJIHUAZHUANGTAI。MSPFALINGMINDUJIAOGAO,YINGYONGFANWEIGUANG。

硫化测序PCRBSP原理:

大乐透复式计算器     JIEHEZHONGYALIUSUANYANDECEXUFASHIYIZHONGLINGMINDENENGZHIJIEJIANCEFENXIJIYINZUDNAJIAJIHUAMOSHIDEFANGFA。ZHONGYALIUSUANYANCHULIHOU,YONGZHENDUIGAIBIANHOUDEDNAXULIESHEJITEYIXINGYINWUBINGJINXINGJUHEMEILIANSHIFANYING(PCR)。 PCRCHANWUZHONGYUANXIANFEIJIAJIHUADEBAOMIDINGWEIDIANBEIXIONGXIANMIDINGSUOTIDAI,ERJIAJIHUADEBAOMIDINGWEIDIANBAOCHIBUBIAN。PCRCHANWUKELONGHOUJINXINGCEXU。TONGGUOZHEIGEFANGFANENGDEDAOTEDINGWEIDIANZAIGEGEJIYINZUDNAFENZIZHONGDEJIAJIHUAZHUANGTAI。

1)客户需提供资料:

1.JINKENENGXINXIANHEZULIANGDEZUZHI(≥50mg)、XIBAOYANGPIN(≥106)HUOQUANXUE(≥300μl)

2.直接提供纯化好的DNA(≥5μg/样品)

4)最终提供客户资料:

1.完整的实验过程报告;
大乐透复式计算器2.数据分析结果(发表级图片形式)。

5)示例展示:


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