大乐透复式计算器

大乐透复式计算器 QUANGUOFUWUREXIAN:

0510-81608116

COIP检测

1)技术简介:

MIANYIGONGCHENDIAN(Co-Immunoprecipitation,Co-IP)SHIYANJIUDANBAIZHIYIDANBAIZHIXIANGHUZUOYONGDECHANGYONGJISHU,TONGCHANGYONGYUCEDINGLIANGZHONGYIZHIDANBAIZHINENGFOUZAIXIBAONEIJIEHECHANSHENGXIANGHUZUOYONG,YIJIYONGYUQUEDINGYUMOUZHONGTEDINGDANBAIZHIJUYOUXIANGHUZUOYONGDEWEIZHIDANBAIZHI。GAIFADEYOUDIANSHIDANBAIZHICHUYUTIANRANZHUANGTAI,DANBAIZHIDEXIANGHUZUOYONGKEYIZAITIANRANZHUANGTAIXIAJINXING,KEYIFENLIDEDAOTIANRANZHUANGTAIXIAXIANGHUZUOYONGDEDANBAIZHIFUHETI。

Co-IPDEYUANLIYUMIANYICHENDIAN(Immunoprecipitation, IP)DAZHIXIANGSI,DOUYOUTEYIKANGTIYUDAIJIANYANGPINZHONGXIANGYINGDETEYIKANGYUANJIEHEXINGCHENGKANGYUANYIKANGTIMIANYIFUHEWU,RANHOUFUHEWUXIFUYUGUHUALEDANBAIAHUOGDEZHICHIWUSHANG(DANBAIAHUOGJUYOUXIFUKANGTIDENENGLI),XIANGYINGDEKANGYUANFENZIYETONGSHIBEIXIFU。MIANYIFUHEWUBEIXIFUDAOZHICHIWUSHANGDEGUOCHENGJIWEICHENDIAN(Precipitation)。MEIYOUBEICHENDIANDEDANBAIZHISUIZHEHUANCHONGYEDELIUXIERBEICHUQU。DANZAIMIANYIGONGCHENDIANZHONG,YUBAKANGYUANYIQIBEICHENDIANDEHAIYOUBAKANGYUANDEXIANGHUZUOYONGDANBAIZHI,JISUIZHEKANGTIBEIXIFUYUGUHUALEDANBAIAHUOGDEZHICHIWUSHANG,XIANGYINGDEKANGYUANJIQIXIANGHUZUOYONGDANBAIZHIYETONGSHIBEICHENDIAN。ZUIHOU,CAIYONGXIANGHUZUOYONGDANBAIZHIDETEYIKANGTIJINGWestern BlotJIANCE,YIZHENGSHIERZHECUNZAIXIANGHUZUOYONG。

2)实验步骤/流程图:

ZONGDANBAITIQU:

大乐透复式计算器 XIBAOYANGPINZHUNBEI:3000rpmLIXIN5minSHOUJIXIBAO,PBSPIAOXI,JIARUSHILIANGmodified RIPA Buffer(HANDANBAIMEIYIZHIJI),BINGSHANGLIEJIE30min,RANHOUYU4℃,12000rpmLIXIN20minQUSHANGQING;

ZUZHIYANGPINZHUNBEI:QUSHILIANGZUZHI,PBSPIAOXI,JIA5BEITIJIYULENGDEmodified RIPA BufferYUBINGSHANGCHONGFENYUNJIANG,YUE5min,RANHOUYU4℃,12000rpmLIXIN20minQUSHANGQING;

大乐透复式计算器 QUSHAOLIANGSHANGQINGYIBEIWesternBlotFENXI,SHENGYUSHANGQINGJIA1μgXIANGYINGDEKANGTI,4℃HUANMANYAOHUANGFUYUGUOYE;

proteinA beads YUXI:QU10μL protein A beads,YONGSHILIANGmodifiedRIPA BufferXI3CI,MEICI3000rpmLIXIN3min,ZAIYONGmodified RIPA BufferDIAOZHENGCHENGTIJIBIWEI50%HUNXUANYE;

JIANGYUXIDE10μLprotein A beadsJIARU2)ZHONGHEKANGTIFUYUGUOYEDEmodified RIPA Buffer,4℃HUANMANYAOHUANGFUYU2-4h,SHIKANGTIYUprotein A beadsCHONGFENOULIAN;

大乐透复式计算器 4℃,3000rpmLIXIN3min,QISHANGQING,1mLmodified RIPA BufferXI3-4CI;JIARU15μLDE2×SDSSHANGYANGHUANCHONGYE,FEISHUIZHU5FENZHONG;

WesternBlotting分析,确定结合蛋白。

3)客户需提供资料:

  样本以及对应的抗体

4)最终提供客户资料:

  详细实验报告,含所需试剂耗材、仪器设备、操作过程

5) 示例展示:


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  • 技术服务热线:18015308936
  • 仪器服务热线:13656167112
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